RNA Analysis

Illumina™ RNA Library Preparation

Total RNA library preparation incl. depletion of rRNA

Goal: sequencing of all coding and non coding RNA within a sample after rRNA removal. Additionally, mitochondrial RNA may also be removed. 

• Human
• Mouse
• Rat
• Gram-positive bacteria
  
• Gram-negative bacteria
• Plant Leaf/Seed/Root

mRNA library preparation

Goal: sequencing of the massenger RNA which codes for proteins. Identification of over and under expressed genes, novel transcripts and fusion products within the transcriptome

• mRNA with stranded orientation

Single cell and low input RNA library preparation

Goal: sequencing of full-length transcripts from a single cell or low input RNA.

• starting from 2 pg input RNA

small RNA library preparation

Goal: sequencing of small and miRNAs wich are expressed within a sample to identify post-transcriptional gene silencing and regulation.

• miRNA libraries
• small RNA libraries

Illumina™ RNA Library Sequencing

StarSEQ offers sequencing of RNA libraries with Illumina’s MiSeq and NextSeq 2000

NextSeq 2000 Sequencing

• Read length: 1×50 nt, 2×50 nt, 1×150 nt, 2×100 nt and 2×150 nt possible
• Read amounts: up to 2000 M reads per run
  

MiSeq Sequencing

• Read length: 1x 50 nt, 2×75 nt, 1×150 nt, 2x 150 nt, 2x 250 nt, 2x 300 nt
• Read amounts: only full flow cells from 2 mio reads to 50 mio reads

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