Life Science/Next Generation Sequencing/

RNA Analysis

Illumina™ RNA Library Preparation

 

Total RNA library preparation incl. depletion of rRNA

Goal: sequencing of all coding and non coding RNA within a sample after rRNA removal. Additionally, mitochondrial RNA may also be removed. 

  • Human
  • Mouse
  • Rat
  • Gram-positive bacteria
  • Gram-negative bacteria
  • Plant Leaf/Seed/Root

mRNA library preparation

Goal: sequencing of the massenger RNA which codes for proteins. Identification of over and under expressed genes, novel transcripts and fusion products within the transcriptome

  • mRNA with stranded orientation

small RNA library preparation

Goal: sequencing of small and miRNAs wich are expressed within a sample to identify post-transcriptional gene silencing and regulation.

  • miRNA libraries
  • small RNA libraries

Illumina™ RNA Library Sequencing

 

StarSEQ offers sequencing of RNA libraries with Illumina’s MiSeq and NextSeq 500

 

NextSeq Sequencing

  • Read length: 1×75 nt, 2×75 nt, 1×150 nt, 2×150 nt possible
  • Read amounts: minimum amount is 25 mio reads, custom amounts possible

MiSeq Sequencing

  • Read length: 1x 50 nt, 2×75 nt, 1×150 nt, 2x 150 nt, 2x 250 nt, 2x 300 nt
  • Read amounts: only full flow cells from 1 mio reads to 50 mio reads

 

 

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