RNAseq Promotion:

HIGH THROUGHPUT GENE EXPRESSION PROFILING

HIGH TROUGHPUT GENE EXPRESSION PROFILING FOR 6, 12, 24, 36, 40 and 80 SAMPLES

 

Flexible voucher option: Save your funds and budgets!

Buy now and perform this or any other project within 12 months.

Changes on project design possible at any time.

 

Service includes:

 

  • mRNA isolation from total RNA for 6, 12, 24,36, 40 and 80 samples
  • NEBNext® Ultra™ II Directional RNA library preparation
  • Quantification and QC of library
  • Sequencing of 1 x 50 nt or 2 x 150 nt with Illumina NextSeq 2000™
  • Data delivery of FASTQ files via download

Output:

 

  • 25 mio SE reads¹ (1 x 50 nt, 1.25 Gb) for only 192,50 €/sample, 80 samples*
  • 25 mio SE reads¹ (1 x 50 nt, 1.25 Gb) for only 222,50 €/sample, 36 samples*
  • 25 mio SE reads¹ (1 x 50 nt, 1.25 Gb) for only 232,50 €/sample, 24 samples*
  • 25 mio SE reads¹ (1 x 50 nt, 1.25 Gb) for only 247,50 €/sample, 12 samples*
  • 50 mio SE reads¹ (1 x 50 nt, 2,5 Gb) for only 280,- €/sample, 40 samples*
  • 50 mio SE reads¹ (1 x 50 nt, 2,5 Gb) for only 300,- €/sample, 36 samples*
  • 50 mio SE reads¹ (1 x 50 nt, 2,5 Gb) for only 310,- €/sample, 24 samples*
  • 50 mio SE reads¹ (1 x 50 nt, 2,5 Gb) for only 325,- €/sample, 12 samples*
  • 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 230,50 €/sample, 40 samples*
  • 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 240,50 €/sample, 36 samples*
  • 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 250,50 €/sample, 24 samples*
  • 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 265,50 €/sample, 12 samples*
  • 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 275,50 €/sample, 6 samples*
  • 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 316,- €/sample, 40 samples*
  • 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 336,- €/sample, 36 samples*
  • 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 346,- €/sample, 24 samples*
  • 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 361,- €/sample, 12 samples*
  • 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 371,- €/sample, 6 samples*

¹ Depending on the nature of starting material and according to Illumina specifications the data output can vary 5-10%

*Samples must be delivered as batch

Optional:

 

  • Bioinformatic data analysis 1: RNA-Seq Alignment to reference genomes² (STAR aligner, DRAGEN, Manta, Salomon, Strelka2) from 70,- €/sample
  • Bioinformatic data analysis 2: Pairwise comparison to identify differentially regulated genes with DESeq2 or DRAGEN workflow from 90,- €/comparison
  • Bioinformatic data analysis 3: RNA-Seq Alignment to non-standard genomes (HISAT2, StringTie) from 100,- €/sample
  • Bioinformatic data analysis 4: Differential expression of novel and reference transcripts with Ballgown workflow from 120 €/sample
  • Bioanalyzer™ check of RNA samples: starting from 15,- €/sample
  • Qubit™ check with RNA assay: starting from 10,- €/sample
  • rRNA depletion: starting from 80,- €/sample

²Available reference genomes include: H. sapiens hg19, H. sapiens GRCh38Decoy, M. musculus mm10, M. musculus UCSC mm9, R. norvegicus rn5, D. melanogaster dm3, B. taurus bosTau6, S. scrofa susScr3, G. gallus galGal4, D. rerio danRer7, C. elegans ce10, Z. mays AGPv3, A. thaliana TAIR10, O. sativa japonica IRGSP-1.0, S. cerevisiae R64-1-1

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