RNAseq Promotion:
HIGH THROUGHPUT GENE EXPRESSION PROFILING

HIGH TROUGHPUT GENE EXPRESSION PROFILING STARTING FROM 6 SAMPLES
Flexible voucher option: Save your funds and budgets!
Buy now and perform this or any other project within 12 months.
Changes on project design possible at any time.
Service includes:
- mRNA isolation from total RNA starting from 6 samples
- NEBNext® Ultra™ II Directional RNA library preparation
- Quantification and QC of library
- Sequencing of 1 x 50 nt or 2 x 150 nt with Illumina NextSeq 2000™
- Data delivery of FASTQ files via download

Price example:
- 25 mio SE reads¹ (1 x 50 nt, 1.25 Gb) for only 212,- €/sample, 40 samples*
- 50 mio SE reads¹ (1 x 50 nt, 2,5 Gb) for only 315,- €/sample, 40 samples*
- 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 210,- €/sample, 48 samples*
- 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 220,- €/sample, 36 samples*
- 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 230,- €/sample, 24 samples*
- 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 245,- €/sample, 12 samples*
- 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 255,- €/sample, 6 samples*
- 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 295,- €/sample, 48 samples*
- 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 305,- €/sample, 36 samples*
- 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 315,- €/sample, 24 samples*
- 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 330,- €/sample, 12 samples*
- 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 340,- €/sample, 6 samples*
¹ Depending on the nature of starting material and according to Illumina specifications the data output can vary 5-10%
*Samples must be delivered as batch

Optional:
- Bioinformatic data analysis 1: RNA-Seq Alignment to standar genomes and non-standard genomes² (STAR aligner, DRAGEN) from 70,- €/sample
- Bioinformatic data analysis 2: Pairwise comparison to identify differentially regulated genes with DESeq2 or DRAGEN workflow from 80,- €/comparison
- Bioanalyzer™ check of RNA samples: starting from 15,- €/sample
- Qubit™ check with RNA assay: starting from 10,- €/sample
- rRNA depletion: starting from 80,- €/sample
²Available reference genomes include: H. sapiens hg19, H. sapiens GRCh38Decoy, M. musculus mm10, M. musculus UCSC mm9, R. norvegicus rn5, D. melanogaster dm3, B. taurus bosTau6, S. scrofa susScr3, G. gallus galGal4, D. rerio danRer7, C. elegans ce10, Z. mays AGPv3, A. thaliana TAIR10, O. sativa japonica IRGSP-1.0, S. cerevisiae R64-1-1
