RNAseq Promotion:

LIBRARY, SEQUENCING-RUN AND BIOINFORMATICS

LIBRARY, SEQUENCING-RUN AND BIOINFORMATICS

 

Flexible voucher option: Save your Funds and Budgets!

Buy now and perform this or any other project within 12 months.

Changes on project design possible at any time!

 

Service includes:

 

  • Quality and quantity check of total RNA
  • mRNA isolation from total RNA starting from 1 sample
  • NEBNext® Ultra™ II Directional RNA library preparation
  • Quantification and QC of library
  • Sequencing of 1 x 75 nt/2 x 150 nt with Illumina Next Seq500™
  • Bioinformatic data analysis 1: RNA-Seq Alignment to reference genomes* (STAR aligner, Manta, Salomon, Strelka2)
  • Data delivery of FASTQ files via download

Output:

 

  • 10 mio PE reads** (2 x 150 nt, 1.5 Gb) for only 360,- €/sample
  • 25 mio PE reads** (2 x 150 nt, 3.75 Gb) for only 690,- €/sample
  • 30 mio PE reads** (2 x 150 nt, 4.5 Gb) for only 760,- €/sample
  • 50 mio PE reads** (2 x 150 nt, 7.5 Gb) for only 890,- €/sample
  • 50 mio SE reads** (1 x 75 nt, 3.75 Gb) for only 750,- €/sample

Optional:

 

  • Bioinformatic data analysis 2: Pairwise comparison to identify differentially regulated genes with DESeq2 workflow from 120,- €/comparison
  • Bioinformatic data analysis 3: RNA-Seq Alignment to non-standard genomes (HISAT2, StringTie) from 100,- €/sample
  • Bioinformatic data analysis 4: Differential expression of novel and reference transcripts with Ballgown workflow from 120 €/sample
  • rRNA depletion using gold standard Ribo-Zero Gold rRNA removal Kit from Illumina: 150,- €/sample

 

*Available reference genomes include:

H. sapiens hg19, H. sapiens GRCh38Decoy, M. musculus mm10, M. musculus UCSC mm9, R. norvegicus rn5, D. melanogaster dm3, B. taurus bosTau6, S. scrofa susScr3, G. gallus galGal4, D. rerio danRer7, C. elegans ce10, Z. mays AGPv3, A. thaliana TAIR10, O. sativa japonica IRGSP-1.0, S. cerevisiae R64-1-1

 

**depending on the nature of starting material and according to Illumina specifications the data output can vary 5-10%

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