Industries/

Genotyping Services

Mouse and Rat Genotyping

 

StarSEQ offers highly efficient and high throughput genotyping of your knockout/-in transgenic strains with state-of-the-art equipment and techniques. Our service includes all steps of the genotyping process, from DNA extraction to a convincing genotyping report. Our service covers single and multiplex PCRs. Furthermore we offer restriction enzyme digestion, RT-PCR and sequencing of SNPs and Indels. For PCR product analysis a high-throughput-capillary gel electrophoresis, QIAxcel-Systems (Quiagen™), is used. Deletions, insertions and SPNs are sequenced with a 3730 DNA Analyzer (Applied Biosystems™).

 

 

Additionally we can offer you a PCR assay validation and development service if you want to genotype your mice or rats by yourself. Do not waste your time with unsuccessful attempts. We have over 10 years of experience in developing high reliable PCR assays.

 

For quotations and details, please contact us using the form below.

 

1. DNA ext raction from low or high throughput sample amounts: tissue, tail or ear biopsy

2. PCR amplification – an initial one-time setup fee is required for new mouse genotyping assays. New assays may take one or two weeks to implement.

3. High Throughput Multicapillary Electrophoresis Using Qiagen QIAxcel System

4. Documentation with Electropherogram and Gel Image

5. Genotyping Analysis with Bio Calculator Software, Examination by Senior Scientist and Report

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