Life Science/Next Generation Sequencing/

DNA Analysis

Incredible 16S and ITS Microbiome Analysis Promotion

 

“All In One Microbiome” Packages: High Throughput Amplicon Generation, Sequencing And Analysis

 
from 32 € /sample
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Illumina™ DNA Library Preparation

 

 

Single- and paired-end libraries from:

 

Goal: discovery of mutations, single-nucleotide polymorphisms (SNPs), rearrangements or analyzing DNA-protein interactions through re-sequencing or de novo sequencing.

 

  • genomic DNA
  • ChIPed DNA samples
  • plasmids

 


 

Special libraries:

 

  • microbiome amplicon generation (V4; V4-V5; ITS), up to 384 indices possible
  • mate-pair library preparation
  • enrichment of human exome or custom regions
  • library QC
  • highly multiplexed PCR amplicon libraries

 


 

In vitro transposition based libraries from:

 

Goal: discovery of mutations, single-nucleotide polymorphisms (SNPs), rearrangements through re-sequencing or de novo sequencing.

 

  • only 1 ng DNA (small genomes, plasmids, amplicons)
  • 50 ng DNA (large genomes)

 


 

Further options:

 

Goal: adapt library size to read length and sequencing strategy

 

  • size selection from 200-800 bp insert size
  • up to 96 indices possible

Illumina™ DNA Library Sequencing

 

StarSEQ offers sequencing of DNA libraries with Illumina’s MiSeq™ and NextSeq 500™

 

NextSeq Sequencing

 

  • Read length: 1×75 nt, 2×75 nt, 1×150 nt, 2×150 nt
  • Read amounts: minimum amount is 25 mio reads, custom amounts possible

 


MiSeq Sequencing

 

  • Read length: 1x 50 nt, 2×75 nt, 1×150 nt, 2x 150 nt, 2x 250 nt, 2x 300 nt
  • Read amounts: only full flow cells. 1 mio reads for nano flow cell V2; 4 mio reads for micro flow cell V2; 12-15 mio reads for full flow cell V2 single end; 24-30 mio reads for full flow cell V2 paired end; 22-25 mio reads for full flow cell V3 single end and 44-50 mio reads for full flow cell V3 paired end

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