Life Science/Next Generation Sequencing/

DNA Analysis

Bacterial-seq promotion!

 

Sequencing of bacterial genomes to 100 fold coverage

 

• Quantification of gDNA
• DNA sequencing library preparation
• Enzymatic fragmentation
• Only 10-100 ng of gDNA required
• Sequencing preparation tagged with barcoded adapters for multiplexing
• Library check
• Sequencing preparation

• Paired-end sequencing, read length 150 nt, NextSeq 2000™
• Qubit/dilution
• Data storage and conversion
• Index managemt and de-multiplexing
• Data delivery via download

Download Flyer

1 Mb genome size, 650 k reads: starting from 170 €

 

2 Mb genome size, 1.5 M reads: starting from 175 €

 

3 Mb genome size, 2 M reads: starting from 180 €

 

5 Mb genome size, 3.3 M reads: starting from 190 €

 

8 Mb genome size, 5.3 M reads: starting from 200 €

 

10 Mb genome size, 6.6 M reads: starting from 205 €

 

15 Mb genome size, 10 M reads: starting from 220 €

 

Final pricing is depending on the amount of samples you send as batch.

Optional: Bioinformatic services

  1. De novo assembly to contigs using SPAdes¹ Genome Assembler: 40 €/sample (1-3 Mb), 50 €/sample (5-15 Mb) ¹Lapidus A., Antipov D., Bankevich A., Gurevich A., Korobeynikov A., Nurk S., Prjibelski A., Safonova Y., Vasilinetc I., Pevzner P. A. New Frontiers of Genome Assembly with SPAdes 3.0. (poster), 201; Bankevich A., Nurk S., Antipov D., Gurevich A., Dvorkin M., Kulikov A. S., Lesin V., Nikolenko S., Pham S., Prjibelski A., Pyshkin A., Sirotkin A., Vyahhi N., Tesler G., Alekseyev M. A., Pevzner P. A. SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing. Journal of Computational Biology, 2012;
  2. Bacterial analysis – identifying antimicrobial resistance genes + MLST analysis + search for plasmid replicons + plasmid MLST analysis: starting from 60 €/sample
  3. Prokka² Genome Annotation – annotation of genes and identifying coding sequences in prokaryotic genomes: starting from 40 €/sample (²Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics 2014 Jul 15;30(14):2068-9. PMID:24642063)

DNAseq promotion and voucher option!

Buy now and perform this or any other project within 12 months

 

DNA Sequencing:

• Quantification of gDNA
• DNA sequencing library preparation
• Enzymatic fragmentation
• Only 10 ng of gDNA required
• Sequencing preparation tagged with barcoded adapters for multiplexing
• Size selection to a major fragment size of 200-800 bp
• Library check
• Sequencing preparation

• Paired-end sequencing, read length 150 nt, NextSeq 2000™
• Qubit/dilution
• Data storage and conversion
• Index managemt and de-multiplexing
• Data delivery via download

  • Price for 1 sample (25 mio reads / 3.75 Gb) 285.50 €/sample
  • Price for 1 sample (50 mio reads / 7.5 Gb) 385,- €/sample
  • Price for 6 samples (25 mio reads / 3.75 Gb) 255.50 €/sample
  • Price for 6 samples (50 mio reads / 7.5 Gb) 351,- €/sample
  • Price for 12 samples (25 mio reads / 3.75 Gb) 245.50€/sample
  • Price for 12 samples (50 mio reads / 7.5 Gb) 341,- €/sample
  • Price for 24 samples (25 mio reads / 3.75 Gb) 225.50 €/sample
  • Price for 24 samples (50 mio reads / 7.5 Gb) 321,- €/sample

Incredible 16S and ITS Microbiome Analysis Promotion

 

“All In One Microbiome” Packages: High Throughput Amplicon Generation, Sequencing And Analysis

 
from 31.20 € /sample
Find Out More

Illumina™ DNA Library Preparation

 

 

Single- and paired-end libraries from:

 

Goal: discovery of mutations, single-nucleotide polymorphisms (SNPs), rearrangements or analyzing DNA-protein interactions through re-sequencing or de novo sequencing.

 

  • genomic DNA
  • ChIPed DNA samples
  • plasmids

 


 

Special libraries:

 

  • microbiome amplicon generation (V4; V4-V5; ITS), up to 384 indices possible
  • mate-pair library preparation
  • enrichment of human exome (Agilent Human All Exon V7) or custom regions
  • enrichment of non-human exomes (Agilent Mouse All Exon, Bovine All Exon, Zebrafish All Exon)
  • library QC
  • highly multiplexed PCR amplicon libraries

 


 

In vitro transposition based libraries from:

 

Goal: discovery of mutations, single-nucleotide polymorphisms (SNPs), rearrangements through re-sequencing or de novo sequencing.

 

  • only 1 ng DNA (small genomes, plasmids, amplicons)
  • 50 ng DNA (large genomes)

 


 

Further options:

 

Goal: adapt library size to read length and sequencing strategy

 

  • size selection from 200-800 bp insert size
  • up to 96 indices possible

Illumina™ DNA Library Sequencing

 

StarSEQ offers sequencing of DNA libraries with Illumina’s MiSeq™ and NextSeq 2000™

 

NextSeq 2000 Sequencing

 

  • Read length: 1×50 nt, 2×50 nt, 1×100 nt, 2×100 nt, 2×150 nt
  • Read amounts: minimum amount is 25 mio reads, custom amounts possible

Download NextSeq Sequencing Order Form

MiSeq Sequencing

 

  • Read length: 1x 50 nt, 2×75 nt, 1×150 nt, 2x 150 nt, 2x 250 nt, 2x 300 nt
  • Read amounts: only full flow cells. 1 mio reads for nano flow cell V2; 4 mio reads for micro flow cell V2; 12-15 mio reads for full flow cell V2 single end; 24-30 mio reads for full flow cell V2 paired end; 22-25 mio reads for full flow cell V3 single end and 44-50 mio reads for full flow cell V3 paired end
Download MiSeq Sequencing Order Form
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