Life Science/

Sanger Sequencing

U-mix

 

 

You mix DNA and primer – we sequence, purify, load and analyse the samples. You receive the complete unedited sequence. Here read lengths of over 1100 bp are possible.

StarSEQ® can only offer the unbelievable price because of its high degree of automation during the whole sequencing process. Please follow the recommended primer and DNA concentration, since these are essential for the success of the sequencing reaction. Unsuccessful reactions will also be billed. Abbreviation and numbering on top of the lid of your sample tubes must be identical to order form!

  1. Login.
  2. Fill in the order form electronically.
  3. Submit the order form.
  4. Receive the completed order form as PDF by e-mail and popup window.
  5. Print order form and sign it.
  6. Label the reaction containers legibly on the lids with an indelible black pen.
  7. Mix the necessary amounts of DNA with primer, fill up to a total volume of 7 µl (if needed with water) (Please note the tips “… you will need”!)
  8. Protect the reaction containers with a box or something similar and send together with order form in a padded envelope to:

 

StarSEQ GMBH, Johann-Joachim-Becher-Weg 30a, 55099 Mainz, Germany

For any questions or requests on your samples please contact us with your complete personal details, date of received results and the abbreviation of your samples under the following E-mail address: u-mix@starseq.com

 

… you will need:

 

DNA: dissolved in water or TrisHCl (10mM), pH 7-8

 

Amounts:
PCR product: < 200 bp: 50 ng; 200 – 300 bp: 60 ng; 301 – 400 bp: 80 ng; 401 – 500 bp: 100 ng; 501 – 600 bp: 120 ng; 601 – 700 bp: 140 ng; 701 – 800 bp: 160 ng; 801 – 900 bp: 180 ng; 901 – 1 kb: 200 ng
Plasmid DNA: <3,5 kb: 350 ng; 3,5 – 4,5 kb: 400 ng; 4,6 – 5,5 kb: 450 ng; 5,6 – 6,5 kb: 500 ng; 6,6 – 7,5 kb: 550 ng; 7,6 – 10 kb: 600 ng; > 10 kb: 700 ng
Cosmid DNA, PACs, BACs: >1 µg

 

We recommend to measure the DNA concentration with fluorescent assay or on a agarose gel.

 

Primer: 10 pmol, i.e. 1 µl of a 10 µMolar primer solution
Melting temperature: 52 – 58°C

Optimal length: 18 – 25mer

Primer base composition: G or C should be at the 3’ end, try to avoid more than 3 G’s or C’s at the end and 4 identical bases in a row.

Tube/Plate: 200 µl PCR tubes with flat lids (e. g. Starlab, Art. Nr. I 1402-8100 or similar); Close only, no Parafilm. Always fill plates from A1 to H1, A2 to H2, A3 to H3… Samples in Plate: Its your choice (must be between 48 and 96 samples)!
Label: legible, with indelible black pen (e. g. Staedtler permanent lumocolor; Art. Nr. 318-9; EAN 40 07817 304563 or similar). Note your abbreviation on the lids and number them continuously. Please do not use special characters for the sample names like / [ \ } & % , ; : . ä ö ü ? ß ( ) ” ‘ # + * @ µ and blanks. Underscores and hyphen could be used.

Dispatch: Protect the tube with suitable firm boxes or bubble wrap and send them in stable tearproof padded envelope.

 

*All quoted prices are without tax.

49 – 96 U-mix samples in plate
3 €
(per sequence read)
batch from 20 U-mix samples and up
4,70 €
(per Sequence Read)
batch of 1-19 U-mix samples
4,90 €
(per Sequence Read)

Existing customers: Login to MyAccount or order

 

Registration: new customers please click here to register. After you have submitted your registration you will receive a confirmation email with a link to finalize the registration process. You will then receive another email with your login details. If you do not receive any email please check your spam folder.

 

V-mix user please click here!

 

Attention: Webshop and sanger sequencing orders need independent logins because of different security levels.

U-MIX SANGER SEQUENCING PROMOTIONS

U-mix prepaid package: plate of 96 samples
240 €
(per plate)
U-mix prepaid package: 50 samples
175€
(3,50* €/rxn)
U-mix prepaid package: 100 samples
290 €
(2,90* €/rxn)
U-mix prepaid package: 500 samples
1425€
(2,85* €/rxn)
U-mix prepaid package: 1000 samples
2700€
(2,70* €/rxn)

…or simply order by e-mail: info@starseq.com

Cannot be combined with other promotions or discounts. Valid until 31.01.2018.

*All quoted prices are without tax.

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