NEXT GENERATION SEQUENCING
HIGH THROUGHPUT GENE EXPRESSION PROFILING FOR 6, 12 AND 24 SAMPLES
Service includes: - mRNA isolation from total RNA for 6, 12 or 24 samples - NEBNext® Ultra™ II Directional RNA library preparation - Quantification and QC of library - Sequencing of 1x 50 nt, 1x100 nt, 2x50 nt or 2 x 150 nt with Illumina NextSeq 2000
HIGH THROUGHPUT GENE EXPRESSION PROFILING FOR 6, 12 AND 24 SAMPLES
Service includes:
• mRNA isolation from total RNA for 6, 12 or 24 samples
• NEBNext® Ultra™ II Directional RNA library preparation
• Quantification and QC of library
• Sequencing of 2 x 150 nt with Illumina NextSeq 2000
• Data delivery of FASTQ files via download
Optional:
Bioinformatic data analysis 1: RNA-Seq Alignment to reference genomes² (STAR aligner, DRAGEN, Manta, Salomon, Strelka2) from 70,- €/sample
Bioinformatic data analysis 2: Pairwise comparison to identify differentially regulated genes with DESeq2 or DRAGEN workflow from 90,- €/comparison
Bioanalyzer check of RNA samples: 40 € per sample