NEXT GENERATION SEQUENCING

HIGH THROUGHPUT GENE EXPRESSION PROFILING FOR 6, 12 AND 24 SAMPLES

Service includes: - mRNA isolation from total RNA for 6, 12 or 24 samples - NEBNext® Ultra™ II Directional RNA library preparation - Quantification and QC of library - Sequencing of 2x 75 nt or 2 x 150 nt with Illumina NextSeq 500

HIGH THROUGHPUT GENE EXPRESSION PROFILING FOR 6, 12 AND 24 SAMPLES

 

Service includes:
• mRNA isolation from total RNA for 6, 12 or 24 samples
NEBNext® Ultra™ II Directional RNA library preparation
• Quantification and QC of library
• Sequencing of 2 x 150 nt with Illumina NextSeq 500TM
• Data delivery of FASTQ files via download

 

Optional:

Bioinformatic data analysis 1: RNA-Seq Alignment to reference genomes² (STAR aligner, Manta, Salomon, Strelka2) from 70,- €/sample
Bioinformatic data analysis 2: Pairwise comparison to identify differentially regulated genes with DESeq2 workflow from 120,- €/comparison
Bioanalyzer check of RNA samples: 40 € per sample

 

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