NEXT GENERATION SEQUENCING

HIGH THROUGHPUT GENE EXPRESSION PROFILING FOR 6, 12 AND 32 SAMPLES

Service includes: - mRNA isolation from total RNA for 6, 12 or 32 samples - TruSeq stranded RNA library preparation - Quantification and QC of library - Sequencing of 2x 75 nt or 2 x 150 nt with Illumina NextSeq 500

HIGH THROUGHPUT GENE EXPRESSION PROFILING FOR 6 AND 12 SAMPLES

 

Service includes:
• mRNA isolation from total RNA for 6, 12 or 32 samples
• TruSeq stranded RNA library preparation
• Quantification and QC of library
• Sequencing of 2 x 150 nt with Illumina NextSeq 500TM
• Data delivery of FASTQ files via download

 

Optional:

Bioinformatic data analysis 1: RNA-Seq Alignment to reference genomes (TopHat, FPKM/RPKM, Variants, Fusion) 100,- € / sample
Bioinformatic data analysis 2: Pairwise comparison of RNA data to identify differentially regulated genes with Cufflinks workflow 200,- € / comparison
Bioanalyzer check of RNA samples: 40 € per sample

 

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