Important information for U-mix user: StarSEQ® can only offer the unbelievable price because of its high degree of automation during the whole sequencing process. Please follow the recommended primer and DNA concentration, since these are essential for the success of the sequencing reaction. Unsuccessful reactions will also be billed. Abbreviation and numbering on top of the lid of your sample tubes must be identical to order form! 1. Login. 2. Fill in the order form electronically. 3. Submit the order form. 4. Receive the completed order form as PDF by e-mail and popup window. 5. Print order form and sign it. 6. Label the reaction containers legibly on the lids with an indelible black pen. 7. Mix the necessary amounts of DNA with primer, fill up to a total volume of 7 µl (if needed with water) (Please note the tips “… you will need”!) 8. Protect the reaction containers with a box or something similar and send together with order form in a padded envelope to: StarSEQ GMBH, Johann-Joachim-Becher-Weg 30a, 55099 Mainz, Germany
For any questions or requests on your samples please contact us with your copmplete personal details, date of received results and the abbreviation of your samples under the following E-mail address: u-mix@starseq.com
... you will need (Attention, new amounts of DNA required!!!): DNA: dissolved in water or TrisHCl (10mM), pH 7-8 Amounts: PCR product: < 200 bp: 50 ng; 200 – 300 bp: 60 ng; 301 – 400 bp: 80 ng; 401 – 500 bp: 100 ng; 501 – 600 bp: 120 ng; 601 – 700 bp: 140 ng; 701 – 800 bp: 160 ng; 801 – 900 bp: 180 ng; 901 – 1 kb: 200 ng Plasmid DNA: <3,5 kb: 350 ng; 3,5 – 4,5 kb: 400 ng; 4,6 – 5,5 kb: 450 ng; 5,6 – 6,5 kb: 500 ng; 6,6 – 7,5 kb: 550 ng; 7,6 – 10 kb: 600 ng; > 10 kb: 700 ng Cosmid DNA, PACs, BACs: >1 µg Primer: 10 pmol, i.e. 1 µl of a 10 µMolar primer solution Melting temperature: 52 - 60°C Optimal length: 18 - 25mer Tube/Plate: 200 µl PCR tubes with flat lids (e. g. Starlab, Art. Nr. I 1402-8100 or similar); Close only, no Parafilm Always fill plates from A1 to H1, A2 to H2, A3 to H3... Samples in Plate: Its your choice (must be between 48 and 96 samples)! Label: legible, with indelible black pen (e. g. Staedtler permanent lumocolor; Art. Nr. 318-9; EAN 40 07817 304563 or similar). Note your abbreviation on the lids and number them continuously. Please do not use special characters for the sample names like / [ \ } & % , ; : . ä ö ü ? ß ( ) " ' # + * @ µ and blanks. Underscores and hyphen could be used. Dispatch: Protect the tube with suitable firm boxes or bubble wrap and send them in stable tearproof padded envelope.
*All quoted prices are without tax.
Important information for V-mix user: StarSEQ® can only offer the unbelievable price because of its high degree of automation during the whole sequencing process. Please follow the recommended primer and DNA concentration, since these are essential for the success of the sequencing reaction. Unsuccessful reactions will also be billed. Abbreviation and numbering on top of the lid of your sample tubes must be identical to order form! 1. Login. 2. Fill in the order form electronically. 3. Submit the order form. 4. Receive the completed order form as PDF by e-mail and popup window. 5. Print order form and sign it. 6. Label the reaction containers legibly on the lids with an indelible black pen. 7. Please send your samples and primers in 1.5 ml tubes. 8. Protect the reaction containers with a box or something similar and send together with order form in a padded envelope to: StarSEQ GMBH, Johann-Joachim-Becher-Weg 30a, 55099 Mainz, Germany
For any questions or requests on your samples please contact us with your copmplete personal details, date of received results and the abbreviation of your samples under the following E-mail address: v-mix@starseq.com
Recommendations (Attention, new amounts of DNA required!!!): DNA: dissolved in 10-15 µl water or TrisHCl (10mM), pH 7-8. Minimum concentration of your DNA template must be 100 ng/µl! Amounts: purified PCR product: 200 bp: >100 ng; 500 bp: >200 ng 1 kb: >400 ng /sequencing reaction Plasmid DNA: 700 ng - 1 µg /sequencing reaction Cosmid DNA, PACs, BACs: >1 µg /sequencing reaction Primer: 3 µl of a 10 µMolar primer solution for each rxn, standard primer available. You find the list here. For 48- or 96-well plates only one primer should be used for the whole plate. Or, another pair plate for primers should be provided. Melting temperature: 52 - 60°C Optimal length: 18 - 25mer Order form: be sure to fill in sample concentration. StarSEQ will not measure the DNA concentration of your samples. If sample concentration is not specified StarSEQ will take 100 ng/µl as a basis. For V-mix plate please provide sample concentration together with sample name separated with forward slash for each well. Tube: 1.5 ml tubes Label: legible, with indelible black pen (e. g. Staedtler permanent lumocolor; Art. Nr. 318-9; EAN 40 07817 304563). Note your abbreviation on the lids and number them continuously. Please do not use special characters for the sample names like /[\}&%. Underscores and hyphen could be used. Dispatch: Protect the tube with suitable firm boxes and send them in stable padded envelope.
