RNAseq Promotion:

HIGH THROUGHPUT GENE EXPRESSION PROFILING

HIGH TROUGHPUT GENE EXPRESSION PROFILING FOR 6, 12 AND 32 SAMPLES

 

Flexible voucher option: Save your funds and budgets!

Buy now and perform this or any other project within 12 months.

Changes on project design possible at any time.

 

Service includes:

 

  • mRNA isolation from total RNA for 6, 12 or 32 samples
  • NEBNext® Ultra™ II Directional RNA library preparation
  • Quantification and QC of library
  • Sequencing of 1 x 75 nt or 2 x 150 nt with Illumina NextSeq 500™
  • Data delivery of FASTQ files via download

Output:

 

  • 25 mio SE reads¹ (1 x 75 nt, 1.875 Gb) for only 375,- €/sample, 32 samples*
  • 25 mio SE reads¹ (1 x 75 nt, 1.875 Gb) for only 400,- €/sample, 12 samples*
  • 25 mio SE reads¹ (1 x 75 nt, 1.875 Gb) for only 450,- €/sample, 6 samples*
  • 50 mio SE reads¹ (1 x 75 nt, 3.75 Gb) for only 540,- €/sample, 32 samples*
  • 50 mio SE reads¹ (1 x 75 nt, 3.75 Gb) for only 550,- €/sample, 12 samples*
  • 50 mio SE reads¹ (1 x 75 nt, 3.75 Gb) for only 600,- €/sample, 6 samples*
  • 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 375,- €/sample, 32 samples*
  • 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 425,- €/sample, 12 samples*
  • 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 475,- €/sample, 6 samples*
  • 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 560,- €/sample, 32 samples*
  • 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 610,- €/sample, 12 samples*
  • 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 660,- €/sample, 6 samples*

¹ Depending on the nature of starting material and according to Illumina specifications the data output can vary 5-10%

*Samples must be delivered as batch

Optional:

 

  • Bioinformatic data analysis 1: RNA-Seq Alignment to reference genomes² (TopHat, FPKM/RPKM, Variants, Fusion) 100,- €/sample
  • Bioinformatic data analysis 2: Pairwise comparison to identify differentially regulated genes with Cufflinks workflow 200,- €/comparison
  • Bioanalyzer™ check of RNA samples: 40,- €/sample
  • Qubit™ check with RNA assay: 20,- €/sample
  • rRNA depletion using gold standard Ribo-Zero Gold rRNA removal Kit from Illumina: 150,- €/sample

²Available reference genomes include: H. sapiens hg19, H. sapiens hg38, M. musculus mm10, M. musculus UCSC mm9, R. norvegicus rn5, D. melanogaster dm3, B. taurus bosTau6, S. scrofa susScr3, G. gallus galGal4, D. rerio danRer7, C. elegans ce10, Z. mays AGPv3, A. thaliana TAIR10, O. sativa japonica IRGSP-1.0, S. cerevisiae R64-1-1

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