RNAseq Promotion:

HIGH THROUGHPUT GENE EXPRESSION PROFILING

HIGH TROUGHPUT GENE EXPRESSION PROFILING FOR 6 AND 12 SAMPLES

 

Service includes:

 

  • mRNA isolation from total RNA for 6 or 12 samples
  • TruSeq stranded RNA library preparation
  • Quantification and QC of library
  • Sequencing of 2 x 150 nt with Illumina NextSeq 500™
  • Data delivery of FASTQ files via download

Output:

 

  • 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 450,- €/sample, 12 samples
  • 25 mio PE reads¹ (2 x 150 nt, 3.75 Gb) for only 550,- €/sample, 6 samples
  • 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 650,- €/sample, 12 samples
  • 50 mio PE reads¹ (2 x 150 nt, 7.5 Gb) for only 750,- €/sample, 6 samples

¹ Depending on the nature of starting material and according to Illumina specifications the data output can vary 5-10%

Optional:

 

  • Bioinformatic data analysis 1: RNA-Seq Alignment to reference genomes² (TopHat, FPKM/RPKM, Variants, Fusion) 80,- €/sample
  • Bioinformatic data analysis 2: Pairwise comparison to identify differentially regulated genes with Cufflinks workflow 175,- €/comparison
  • Bioanalyzer™ check of RNA samples: 40,- €/sample
  • Qubit™ check with RNA assay: 20,- €/sample

²Available reference genomes include: H. sapiens hg19, H. sapiens hg38, M. musculus mm10, M. musculus UCSC mm9, R. norvegicus rn5, D. melanogaster dm3, B. taurus bosTau6, S. scrofa susScr3, G. gallus galGal4, D. rerio danRer7, C. elegans ce10, Z. mays AGPv3, A. thaliana TAIR10, O. sativa japonica IRGSP-1.0, S. cerevisiae R64-1-1

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