Life Science/Next Generation Sequencing/

Microbiome Analysis

Incredible 16S, 18S and ITS Microbiome Analysis Promotion

 

Flexible Voucher Option: Transfer your Funds and Budget to 2018!

Buy now and perform this or any other project within 12 months.

 

“All in One Microbiome” Packages (16S and ITS): High Throughput Amplicon Generation, Sequencing and Analysis

Package A: 192 samples only 32 € per sample

Package B: 96 samples only 50 € per sample
Package C: 48 samples only 75 € per sample
Package D: 10 samples only 199 € per sample (limited to V4 region)

Microbiome flyer
Chose your option for Amplicon sequencing:

 

Bacterial microbiome analysis:
16S, V4 region, Primer combination: 515F – 806bR*
16S, V4-V5 region, Primer combination: 515F – 924R*

16S, V3-V4 region, Primer combination: 341f – 806bR****

 

Fungal microbiome analysis:
ITS, ITS 1 region, Primer combination: ITS1F – ITS2**

 

Eukaryotes (microbial) analysis:

V9 region, Primer combination: Illumina_Euk_1391f – Illumina_EukBr_1510r, blocking primer optional***

 

Send us your gDNA samples and make use of our “All in One” Service:
• Quality control
• Single step amplicon generation with reduced bias
• Double indexing, quality check, quantification, normalization and pooling of amplicons
• Illumina MiSeq sequencing package A-C: 2 x 300 nt paired-end sequencing with V3 chemistry

• Illumina MiSeq sequencing package D: 2 x 250 nt paired-end sequencing with V2 chemistry nano flow cell
• Output package A-C: 30-40 M reads (including 25 % PhiX to balance the composition of bases)

• Output package D: 750 K reads (including 25 % PhiX to balance the composition of bases)
• De-multiplexing of reads
• Free 16S metagenomics analysis (Illumina App)
• Data delivery via FTP server

 

Applications:
• Environmental Metagenomics (soil, water, air, biofilms, complex organic communities)
• Human or Animal Microbiome (skin, stool, gut, blood, swab)

• Monitoring of animal health (e.g. aternatively or subblementerily to FELASA-Test)
• Sterility Monitoring
• Detection of Contamination
• Monitoring of Biogas Plant
• Biosafety Monitoring
• Food Quality
• Clinical Samples

 

Sequencing of your ready to load 16S/ITS/18S libraries:

• Illumina MiSeq sequencing: 2 x 300 nt paired-end sequencing with V3 chemistry
• Output 30-40 M reads (including 25 % PhiX to balance the composition of bases)
• De-multiplexing of reads
• Free 16S metagenomics analysis (Illumina App)
• Data delivery via FTP server

 

Only 2600 €

 

Primer sequence references:

*Apprill A, McNally S, Parsons R, Weber L. 2015. Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton. Aquat Microb Ecol 75:129–137. Parada AE, Needham DM, Fuhrman JA. Every base matters: assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples. Environ Microbiol. 2016;18: 1403–1414.

*Parada, A. E., Needham, D. M., & Fuhrman, J. A. (2016). Every base matters: assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples. Environmental Microbiology, 18(5), 1403–1414. https://doi.org/10.1111/1462-2920.13023

*Walters, W., Hyde, E. R., Berg-Lyons, D., Ackermann, G., Humphrey, G., Parada, A., … Knight, R. (2016). Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribed Spacer Marker Gene Primers for Microbial Community Surveys. mSystems, 1(1), e00009-15. https://doi.org/10.1128/mSystems.00009-15

**White, T. J., T. Bruns, S. Lee, and J. W. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp. 315-322 In: PCR Protocols: A Guide to Methods and Applications. Academic Press, New York, NY. Gardes, M., and T. D. Bruns. 1993. ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts. Mol. Ecol. 2: 113-118.

***Amaral-Zettler, L. A., McCliment, E. A., Ducklow, H. W., & Huse, S. M. (2009). A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes. PLOS ONE, 4(7), e6372. Retrieved from https://doi.org/10.1371/journal.pone.0006372

**** Klindworth A., Pruesse E., Schweer T., Peplies J., Quast C., Horn M., et al. . (2013). Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies. Nucleic Acids Res. 41, 1–11. 10.1093/nar/gks808

****Takahashi S, Tomita J, Nishioka K, Hisada T, Nishijima M. Development of a Prokaryotic Universal Primer for Simultaneous Analysis of Bacteria and Archaea Using Next-Generation Sequencing. Bourtzis K, ed. PLoS ONE. 2014;9(8):e105592. doi:10.1371/journal.pone.0105592.

 

see also https://support.illumina.com/downloads/16s_metagenomic_sequencing_library_preparation.html

 

Simply order by e-mail: microbiome@starseq.com

 

You have no time/staff for routine preparation of gDNA from your collected samples? As an additional service we offer also the purification of gDNA from various starting material. Please contact us if you are interested in further service options or other gene regions and we will find YOUR optimal solution!
info@starseq.com

Sequencing of your ready to load 16S/ITS/18S libraries:

• Illumina MiSeq sequencing: 2 x 300 nt paired-end sequencing with V3 chemistry
• Output 30-40 M reads (including 25 % PhiX to balance the composition of bases)
• De-multiplexing of reads
• Free 16S metagenomics analysis (Illumina App)
• Data delivery via FTP server

 

Only 2600 €

Shotgun metagenomic sequencing

 

• Quantification of gDNA
• DNA sequencing library preparation using Agilent SureSelect QXT
• Only 50 ng of gDNA required
• Sequencing preparation tagged with barcoded adapters for multiplexing
• Size selection to a major fragment size of 200-800 bp
• Library check
• Sequencing / 150 nt / PE / 25 mio reads / 3.75 Gb
• Paired-end sequencing, read length 150 nt, Next Seq 500TM
• Sequencing preparation
• Qubit/dilution
• Data storage and conversion
• Index managemt and de-multiplexing
• Data delivery via download

 

Price for 1-4 samples : 650,- €/sample
Price for 5-9 samples: 570,- €/sample
Price from 10 samples: 480,- €/sample

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