Life Science/Next Generation Sequencing/
Microbiome Analysis
Incredible 16S and ITS Microbiome Analysis Promotion
Prepaid option – order now and deliver your sample within the next 12 months:
“All in One Microbiome” Packages: High Throughput Amplicon Generation, Sequencing and Analysis
Package A: 192 samples only 32 € per sample
Package B: 96 samples only 50 € per sample
Package C: 48 samples only 75 € per sample
Package D: 10 samples only 199 € per sample (limited to V4 region)
Bacterial microbiome analysis:
16S, V4 region, Primer combination: 515F – 806bR*
16S, V4-V5 region, Primer combination: 515F – 909R*
Fungal microbiome analysis:
ITS, ITS 1 region, Primer combination: ITS1F – ITS2**
Send us your gDNA samples and make use of our “All in One” Service:
• Quality control
• Single step amplicon generation with reduced bias
• Double indexing, quality check, quantification, normalization and pooling of amplicons
• Illumina MiSeq sequencing package A-C: 2 x 300 nt paired-end sequencing with V3 chemistry
• Illumina MiSeq sequencing package D: 2 x 250 nt paired-end sequencing with V2 chemistry nano flow cell
• Output package A-C: 30-40 M reads (including 25 % PhiX to balance the composition of bases)
• Output package D: 750 K reads (including 25 % PhiX to balance the composition of bases)
• De-multiplexing of reads
• Free 16S metagenomics analysis (Illumina App)
• Data delivery via FTP server
Applications:
• Environmental Metagenomics (soil, water, air, biofilms, complex organic communities)
• Human or Animal Microbiome (skin, stool, gut, blood, swab)
• Monitoring of animal health (e.g. aternatively or subblementerily to FELASA-Test)
• Sterility Monitoring
• Detection of Contamination
• Monitoring of Biogas Plant
• Biosafety Monitoring
• Food Quality
• Clinical Samples
Sequencing of your ready to load 16S/ITS/18S libraries:
• Illumina MiSeq sequencing: 2 x 300 nt paired-end sequencing with V3 chemistry
• Output 30-40 M reads (including 25 % PhiX to balance the composition of bases)
• De-multiplexing of reads
• Free 16S metagenomics analysis (Illumina App)
• Data delivery via FTP server
Only 2600 €
Primer sequence references:
*Apprill A, McNally S, Parsons R, Weber L. 2015. Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton. Aquat Microb Ecol 75:129–137. Parada AE, Needham DM, Fuhrman JA. Every base matters: assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples. Environ Microbiol. 2016;18: 1403–1414.
**White, T. J., T. Bruns, S. Lee, and J. W. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp. 315-322 In: PCR Protocols: A Guide to Methods and Applications. Academic Press, New York, NY. Gardes, M., and T. D. Bruns. 1993. ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts. Mol. Ecol. 2: 113-118.
Simply order by e-mail: microbiome@starseq.com
You have no time/staff for routine preparation of gDNA from your collected samples? As an additional service we offer also the purification of gDNA from various starting material. Please contact us if you are interested in further service options or other gene regions and we will find YOUR optimal solution!
info@starseq.com